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recombinant protein kinase a pka catalytic subunit  (New England Biolabs)


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    Structured Review

    New England Biolabs recombinant protein kinase a pka catalytic subunit
    Recombinant Protein Kinase A Pka Catalytic Subunit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 769 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/catalytic+subunit/PKA/pm42128195-180-0-12
    Average 95 stars, based on 769 article reviews
    recombinant protein kinase a pka catalytic subunit - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Single-molecule identification of full-length proteins with single-amino-acid resolution using nanopores
    Article Snippet: .. 7500 units of cAMP-dependent Protein Kinase (PKA), catalytic subunit (New England Biolabs) was added, and the mixture was incubated for 2 hours at 30°C, under constant shaking at 600 rpm. .. Afterwards, a buffer exchange to 1x PBS (a 10x PBS solution from VWR was diluted 10 times with MilliQ) was performed using a PD10 column (Cytiva) and protein was stored at -20°C until use.

    Article Title: Photonic Crystal Kinase Biosensor
    Article Snippet: .. The catalytic subunit of protein kinase A (New England Biolabs) was added in varying concentrations (0 – 25 U/μL) and incubated at 30 °C for varying times (0 – 8 h). .. The phosphorylation reaction was stopped by inactivating the kinase via submerging the samples in water at 65 °C for 20 min. For experiments in the presence of inhibitor, PKA was pre-incubated for 10 min at 30 °C in 50 mM tris-HCl buffer with 10 mM MgCl2, 100 μM ATP, and varying concentrations of H-89 inhibitor (Cell Signaling Technologies, 0.1 nM– 100 μM).

    Purification:

    Article Title: Electrokinetic detection of single-molecule phosphorylation
    Article Snippet: .. cAMP-dependent Protein Kinase A catalytic subunit (PKAc, 13 μM) and Lambda Protein Phosphatase (λPP, 20 μM) were purchased from New England Biolabs (Ipswich, MA) and used without further purification. .. Peptides (kemptide, PLN20, SP20), both labeled and unlabeled, were custom synthesized by Anaspec (Supplementary Table 1).

    other:

    Article Title: Spatially resolving how cMyBP-C phosphorylation and haploinsufficiency in porcine and human myofibrils affect β-cardiac myosin activity
    Article Snippet: A subset of porcine cardiac myofibrils were treated for 1 h at 4°C on a rotator with 62.5 U/μl of cAMP-dependent protein kinase, catalytic subunit (P600S; New England Biolabs), NEBuffer for protein kinases (B6022S; New England Biolabs), and 200 μM ATP.

    Phospho-proteomics:

    Article Title: HEXIM1 inter-monomer autoinhibition governs 7SK RNA binding specificity and P-TEFb inactivation
    Article Snippet: For even shorter monomeric proteins, 1 kDa MWCO dialysis tubing (for BRext) or desalting column (HiTrap Desalting 5 mL, GE Healthcare; for BR and BR ARM1 ) were used for buffer exchange into 5 mM NH 4 HCO 3 buffer, freeze dried with a lyophilizer and dissolved directly in NMR buffer (50 mM sodium phosphate, pH 6.2, 150 mM KCl, 0.02% NaN 3 , 5% D 2 O). .. Phosphorylation of Hexim1 BR-L-AR was performed utilizing cAMP-dependent protein kinase A catalytic subunit (PKA cat ; NEB P6000S). .. The protein was first buffer-exchanged into NMR buffer (50 mM sodium phosphate, pH 6.2, 150 mM KCl, 0.02% NaN 3 , 5% D 2 O), then concentrated with constant mixing using Amicon devices (Millipore).

    Article Title: HEXIM1 inter-monomer autoinhibition governs 7SK RNA binding specificity and P-TEFb inactivation.
    Article Snippet: .. In vitro phosphorylation by PKA catalytic subunit Phosphorylation of Hexim1 BR-L-AR was performed utilizing cAMP-dependent protein kinase A catalytic subunit (PKAcat; NEB P6000S). ..

    Article Title: HEXIM1 inter-monomer autoinhibition governs 7SK RNA binding specificity and P-TEFb inactivation.
    Article Snippet: For even shorter monomeric proteins, 1kDa MWCO dialysis tubing (for BRext) or desalting column (HiTrap Desalting 5mL, GE Healthcare; for BR and BRARM1) were used for buffer exchange into 5 mM NH4HCO3 buffer, freeze dried with a lyophilizer and dissolved directly in NMR buffer (50mM sodium phosphate, pH 6.2, 150 mM KCl, 0.02% NaN3, 5% D2O). .. In vitro phosphorylation by PKA catalytic subunit Phosphorylation of Hexim1 BR-L-AR was performed utilizing cAMP-dependent protein kinase A catalytic subunit (PKA; NEB P6000S). .. The protein was first buffer-exchanged into NMR buffer (50mM sodium phosphate, pH 6.2, 150 mM KCl, 0.02% NaN3, 5% D2O), then concentrated with constant mixing using Amicon devices (Millipore).

    In Vitro:

    Article Title: HEXIM1 inter-monomer autoinhibition governs 7SK RNA binding specificity and P-TEFb inactivation.
    Article Snippet: .. In vitro phosphorylation by PKA catalytic subunit Phosphorylation of Hexim1 BR-L-AR was performed utilizing cAMP-dependent protein kinase A catalytic subunit (PKAcat; NEB P6000S). ..

    Article Title: HEXIM1 inter-monomer autoinhibition governs 7SK RNA binding specificity and P-TEFb inactivation.
    Article Snippet: For even shorter monomeric proteins, 1kDa MWCO dialysis tubing (for BRext) or desalting column (HiTrap Desalting 5mL, GE Healthcare; for BR and BRARM1) were used for buffer exchange into 5 mM NH4HCO3 buffer, freeze dried with a lyophilizer and dissolved directly in NMR buffer (50mM sodium phosphate, pH 6.2, 150 mM KCl, 0.02% NaN3, 5% D2O). .. In vitro phosphorylation by PKA catalytic subunit Phosphorylation of Hexim1 BR-L-AR was performed utilizing cAMP-dependent protein kinase A catalytic subunit (PKA; NEB P6000S). .. The protein was first buffer-exchanged into NMR buffer (50mM sodium phosphate, pH 6.2, 150 mM KCl, 0.02% NaN3, 5% D2O), then concentrated with constant mixing using Amicon devices (Millipore).



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